Das Lehrgebiet BioVT ist Co-Autor bei einer Publikation in Biotechnology Biotechnology and Bioengineering zum Thema der elektrounterstützen Biokatalyse.

Unspecific peroxygenases (UPOs) have attracted significant scientific interest in the recent years due to their ability to catalyze various types of oxygen transfer reactions, most importantly the hydroxylation of even unactivated C-H bonds, using only hydrogen peroxide as a cosubstrate. Mechanistically they are closely related to cytochrome P450 monooxygenases (P450s), given that the P450 peroxide shunt pathway is identical to the reaction mechanism of UPOs. The native reaction mechanism of P450s, however, usually relies upon a reductase for activation that in turn requires NAD(P)H. This renders the P450 reaction pathway both more complicated and economically less attractive in comparison to the UPO’s “self-sustainability” due to their direct use of cheap H₂O₂. In this work, we sought to investigate an UPO-catalyzed hydroxylation reaction concerning reaction kinetics and enzyme inactivation kinetics while collecting a dataset amenable to modeling with the goal of simulating such a process. Due to the flexibility and simplicity of the system, we chose to concentrate on the electrochemical in situ production of H₂O₂. This combination results in an electro-enzymatic cascade reaction. Understanding, analysis, and control of enzymatic cascade reactions are challenging problems, e.g. due to their complexities, nonlinearities, and delays.

S. Bormann, D. Hertweck, S. Schneider, J.Z. Bloh, R. Ulber, A.C. Spiess, D. Holtmann; Modeling and Simulation-Based Design of Electroenzymatic Batch  Processes catalysed by Unspecific Peroxygenase from A. aegerita; Biotechnology and Bioengineering (2020) https://doi.org/10.1002/bit.27545